Expression Profiling of Inflammatory Breast Cancer Cells Treated with the Novel Histone Deacetylase Inhibitor, CG-1521 (Affymetrix HuGene-1_0)

Studies of gene expression profiles using the whole genome wide microarray analysis in SUM149PT cells (ER-, p53mut) and SUM190PT cells (ER-, p53mut) when treated with 5 or 7.5 uM CG-1521 alone and in combination with 10 nM 17beta-estradiol. Comparisons between each treatment group provides evidence for the dysregulation of genes associated with the spindle assembly checkpoint. Three independent experiments were carried out in SUM149PT and SUM190PT cells, which were treated with vehicle (ethanol/DMSO), 10nM 17beta-estradiol, 5 or 7.5 uM CG-1521, and the combination of 17 beta-estradiol and CG-1521. Total RNA was extracted from cell lysates using QIAGEN RNeasy mini kit after 48h of treatment.

本数据集采用全基因组微阵列分析（whole genome wide microarray analysis），探究SUM149PT细胞（雌激素受体阴性（ER-）、p53突变型（p53mut））与SUM190PT细胞（雌激素受体阴性（ER-）、p53突变型（p53mut））经不同方案处理后的基因表达谱。处理方案包括单独施加5 μM或7.5 μM的CG-1521，以及联合10 nM 17β-雌二醇（17beta-estradiol）进行处理。对各处理组间的基因表达差异进行比较，可为纺锤体组装检验点（spindle assembly checkpoint）相关基因的失调现象提供实验依据。本实验在SUM149PT与SUM190PT细胞中独立重复三次，处理组别涵盖溶剂对照（乙醇/二甲基亚砜（ethanol/DMSO））、10 nM 17β-雌二醇、5 μM或7.5 μM CG-1521，以及17β-雌二醇与CG-1521的联合处理。于处理48小时后，使用QIAGEN RNeasy 微型试剂盒（QIAGEN RNeasy mini kit）从细胞裂解液中提取总RNA。