Drop-Seq analysis of ERCC spike in capture

A bead supsension and a solution of ERCC spike-ins at a concentration of ~100,000 molecules per droplet was used in Drop-Seq, a novel technology for high-throughput single cell mRNAseq Overall design: An estimated 84 beads were selected for amplification.本实验采用磁珠悬浮液与浓度为约每液滴100000个分子的ERCC外源掺入对照（ERCC spike-ins）溶液，应用于Drop-Seq技术——一种用于高通量单细胞mRNA测序的新型技术。实验整体设计：共选取约84个磁珠进行扩增。