RNA seq of hearts from Tead1 global and cardiac-specific knockout mice

12-week-old female CAGGCre-ER-Tead1+/+ (control) and CAGGCre-ER; Tead1flox/flox (inducible global knockout; igKO) mice, and Myh6-MerCreMer-; Tead1flox/flox mice (control) and Myh6-MerCreMer+; Tead1flox/flox (inducible cardiac-specific knockout; icKO) and were subjected to IP injection of tamoxifen (50 mg/kg) for 5 consecutive days. Five days post the last injection, total RNA from the mouse hearts was extracted by TRIzol (Invitrogen). The RNA-seq library was constructed with Ribo-Zero library construction kit (illumina). RNA-sequencing was performed on a HiSeq 3000 system (Illumina) in 50-bp length at Genome Technology Access Center of Washington University.

12周龄雌性CAGGCre-ER-Tead1+/+（对照）、CAGGCre-ER; Tead1flox/flox（诱导型全身性基因敲除，igKO）小鼠，以及Myh6-MerCreMer-; Tead1flox/flox（对照）和Myh6-MerCreMer+; Tead1flox/flox（诱导型心脏特异性基因敲除，icKO）小鼠，均连续5天腹腔注射他莫昔芬（tamoxifen，50 mg/kg）。末次注射后第5天，采用TRIzol试剂（Invitrogen）提取各组小鼠心脏组织的总RNA；使用Ribo-Zero建库试剂盒（illumina）构建RNA测序（RNA-seq）文库；随后于华盛顿大学基因组技术访问中心（Genome Technology Access Center of Washington University），采用Illumina HiSeq 3000测序系统进行50bp读长的RNA测序。