Nucleosome occupancy microarray for WT and jhd2-delete strains though sporulation. Saccharomyces cerevisiae

WT (MMY718) and jhd2∆ (MMY1879) sporulating cell cultures were profiled for global nucleosome occupancy using Affymetrix high-resolution tiling arrays. Overall design: MNase-treated mono-nucleosomes from WT and jhd2∆ cultures in rich media, and sporulation timepoints at 0h, 4h, 6h, 8h, 10h, 12h, and 16h were microarrayed.

本研究采用Affymetrix高分辨率镶嵌芯片（Affymetrix high-resolution tiling arrays），对野生型（WT，菌株编号MMY718）与jhd2基因缺失突变株（jhd2∆，菌株编号MMY1879）的孢子形成细胞培养物的全局核小体占据谱进行了检测分析。实验设计如下：分别提取丰富培养基中培养的野生型与jhd2∆菌株培养物内、经微球菌核酸酶（Micrococcal Nuclease, MNase）处理的单核小体样本，同时收集孢子形成过程中0h、4h、6h、8h、10h、12h及16h共7个时间点的样本，对所有上述样本开展微阵列芯片检测。