Data of "Substrate availability and thermal adaptation constrain the temperature effects on marine bacterioplankton communities in subtropical coastal waters"

Here is a dataset of a study named "Substrate availability and thermal adaptation constrain the temperature effects on marine bacterioplankton communities in subtropical coastal waters". It includes three data files: bacterial abundance, bacterial growth rate, and bacterial results. The R code for analysing these data is also included (i.e., Bacterial_analysis 2605. R). This study is designed to investigate the thermal response of marine bacteria. The experiments were conducted at two contrasting subtropical coastal sites (i.e., Sta. EO and Sta. WE) over a 1-year period. In each experiment, natural bacterial communities (1 μm filtrate) were diluted by particle-free seawater (0.22 μm filtrate) and incubated for about 48h. Bacterial abundance was measured by Flow Cytometry every 4-6 h to obtain complete growth curves and estimate the growth rate. Such experiments were conducted at 7 temperatures, and the thermal traits of bacterial communities (i.e., temperature sensitivity and optimal growth temperature) were estimated using the MTE framework. Therefore, bacterial_abundance.csv contains all data on bacterial abundance, and bacterial_growth_rate.csv contains data on bacterial growth at designated temperatures. bacterial_results.csv is the summary of results for this study, including environmental parameters and experimental outputs (e.g., growth rate, temperature sensitivity).本数据集对应一项题为《亚热带近岸海域底物可利用性与热适应限制温度对海洋浮游细菌群落影响》的研究。数据集包含三个数据文件：细菌丰度、细菌生长速率及细菌实验结果文件，同时附带了用于数据分析的R代码（即Bacterial_analysis 2605.R）。 本研究旨在探究海洋细菌的热响应特征。实验于两个具有显著差异的亚热带近岸站位（即EO站与WE站）开展，实验周期覆盖一整年。每一轮实验中，将天然细菌群落（经1μm滤膜过滤）用无颗粒海水（经0.22μm滤膜过滤）进行稀释后，培养约48小时。每4-6小时采用流式细胞术（Flow Cytometry）测定细菌丰度，以获取完整生长曲线并估算生长速率。本实验共设置7个温度梯度，采用MTE框架估算细菌群落的热适应性参数，即温度敏感性与最适生长温度。 其中，bacterial_abundance.csv包含所有细菌丰度相关数据，bacterial_growth_rate.csv包含指定温度下的细菌生长速率数据；bacterial_results.csv为本次研究的结果汇总文件，涵盖环境参数与实验产出（如生长速率、温度敏感性等）。