Expression data of LncRNA from the rat (Sprague-Dawley) knee articular cartilage at different developmental stages

Age as the primary rise factor could be play an important role in incidence and development of osteoarthritis. A few studies have confirmed some tissue specific lncRNA were associated with development of osteoarthritis. But if age related lncRNA would be involved in pivotal post-transcriptional gene regulation in osteoarthritis is unclear. In view of this, we have an idea that several age-related lncRNA would be screened from the rat knee cartilage at different development ages by lncRNAs Microarray analysis. We used microarrays to detail the global programme of gene expression underlying the rat knee cartilage and identified distinct classes of age-related lncRNA during this process. The rat knee articular cartilage were selected at successive stages of the rat developmental for RNA extraction and hybridization on Affymetrix lncRNA arrays. We sought to obtain homogeneous populations of cartilage at each developmental stage in order to increase the temporal resolution of expression profiles. To that end, we hand-selected cartilage according to the rat developmental stages, i.e. seven time-points: newborn (T0), youth(T1), adult (T2), early-stage elderly(T3) and latter-stage elderly(T4).

年龄作为骨关节炎发生与进展的主要风险因素，在其发病与发展过程中发挥重要作用。已有多项研究证实，部分组织特异性长链非编码RNA（long non-coding RNA, lncRNA）与骨关节炎的进展密切相关。但与年龄相关的lncRNA是否参与骨关节炎关键的转录后基因调控，目前仍不明确。鉴于此，本研究拟通过长链非编码RNA微阵列（lncRNAs Microarray）分析，从不同发育阶段大鼠膝关节软骨中筛选与年龄相关的lncRNA。本研究利用微阵列技术解析大鼠膝关节软骨的全局基因表达谱，并在此过程中鉴定出不同类别的年龄相关lncRNA。我们选取大鼠发育连续阶段的膝关节关节软骨，进行RNA提取并在Affymetrix lncRNA微阵列上完成杂交实验。为提升表达谱的时间分辨率，我们旨在获取各发育阶段的均一化软骨样本。为此，我们根据大鼠发育阶段手动筛选软骨样本，共设置7个时间节点：新生期（T0）、青年期（T1）、成年期（T2）、早期老年期（T3）与晚期老年期（T4）。