Multiomics Combined with Expression Pattern Analysis Reveals the Regulatory Response of Key Genes in Potato Jasmonic Acid Signaling Pathways to Cadmium Stress

Jasmonic acid (JA) is an endogenous phytohormone that
regulates
plant physiological metabolism and stress response processes, either
independently or through hormone crosstalk. Our phytohormone assay
and transcriptome–metabolome analysis revealed the key genes
and metabolites involved in the JA pathway in response to 0–250
μM cadmium (Cd) in potato seedlings. Transcriptome gene set
enrichment and gene ontology analysis indicated that JA-related genes
were significantly enriched. Specifically, members from the StOPR and StJAZ gene families showed pronounced
responses to Cd stress and methyl jasmonate treatment. As a negative
regulatory transcription factor of the JA signaling pathway, StJAZ14 exhibited a decreasing trend under Cd stress. Yeast
two-hybrid assay identified an interaction between StJAZ14 and StBZR1,
which is located on the brassinolide pathway. In addition to unveiling
the critical role of the JA pathway in regulating potato response
to Cd stress, the functional mechanism was preliminarily explored.

茉莉酸（Jasmonic acid, JA）是一类内源植物激素，可独立或通过激素交叉对话调控植物的生理代谢与胁迫响应过程。本研究通过植物激素测定与转录组-代谢组联合分析，明确了马铃薯幼苗响应0~250 μM镉（Cd）胁迫的茉莉酸通路关键基因与代谢物。转录组基因集富集分析与基因本体（Gene Ontology, GO）分析结果显示，茉莉酸相关基因显著富集。具体而言，StOPR与StJAZ基因家族成员对镉胁迫及茉莉酸甲酯处理均表现出显著响应。作为茉莉酸信号通路的负调控转录因子，StJAZ14在镉胁迫下呈现表达量下调趋势。酵母双杂交实验证实，StJAZ14与定位在油菜素内酯通路中的StBZR1存在相互作用。本研究不仅揭示了茉莉酸通路在调控马铃薯响应镉胁迫中的关键作用，还初步探索了其潜在功能机制。