Transcriptomic Profiling of Immune Cell-Enriched Regions in Triple-Negative Breast Cancer Using Targeted RNA Sequencing

This dataset contains targeted RNA sequencing (TempO-Seq) data derived exclusively from immune-enriched regions of triple-negative breast cancer (TNBC) tissues. Spatial transcriptomic profiling was performed on formalin-fixed paraffin-embedded (FFPE) sections, and only areas characterized by immune cell infiltration were microdissected and sequenced.The resulting FASTQ files and normalized gene expression data represent comprehensive gene profiles retrieved from these immune-dominant microenvironments. The goal of this study is to characterize immune gene signatures associated with immune cells and clinical outcome in TNBC.These data contribute to understanding how spatial immune architecture influences prognosis and immune evasion, with implications for immunotherapy stratification in breast cancer. All raw and processed data are freely accessible under this GEO submission.

本数据集仅包含源自三阴性乳腺癌（TNBC）组织免疫富集区域的靶向RNA测序（TempO-Seq）数据。研究人员对福尔马林固定石蜡包埋（FFPE）切片开展空间转录组分析，仅对存在免疫细胞浸润特征的区域进行显微切割并完成测序。所得FASTQ文件与标准化基因表达数据，完整呈现了这些免疫主导微环境中的基因表达谱。本研究旨在表征三阴性乳腺癌中与免疫细胞及临床结局相关的免疫基因特征。上述数据有助于阐明空间免疫架构对预后与免疫逃逸的影响机制，可为乳腺癌免疫治疗分层提供参考依据。所有原始数据与处理后数据均可通过本次基因表达综合数据库（Gene Expression Omnibus）提交项目免费获取。