Data content for main Figures (1-4) and SI in Scientific Reports paper "A handheld platform for target protein detection and quantification using disposable nanopore strips"

<b>Abstract.</b> Accessible point-of-care technologies that can provide immunoassay and molecular modalities could dramatically enhance diagnostics, particularly for infectious disease control in low-resource settings. Solid-state nanopores are simple and durable sensors with low-energy instrumentation requirements. While nanopore sensors have demonstrated efficacy for nucleic acid targets, selective detection and quantification of target proteins from sample background has not been demonstrated. We present a simple approach for electronic detection and quantification of target proteins that combines novel biomolecular engineering methods, a portable reader device and disposable nanopore test strips. The target of interest can be varied by swapping the binding domain on our engineered detection reagent, which efficiently binds in the bulk-phase to the target and subsequently generates a unique signature when passing through the pore. We show modularity of the detection reagent for two HIV antibodies, TNF$\alpha$ and tetanus toxin as targets. A saliva swab-to-result is demonstrated for clinically relevant HIV antibody levels (0.4-20 mg/liter) in under 60 seconds. While other strip-like assays are qualitative, the presented method is quantitative and sets the stage for simultaneous immunoassay and molecular diagnostic functionality within a single portable platform.

<b>摘要.</b> 可实现免疫测定与分子检测的便携床旁检测技术，能够显著提升诊断效能，尤其对资源匮乏地区的传染病防控具有重要意义。固态纳米孔（solid-state nanopores）是一类结构简单、性能耐用的传感器，且对仪器能耗要求较低。尽管纳米孔传感器已被证实可有效检测核酸靶标，但从样本背景中选择性检测并定量靶标蛋白的技术仍未实现。本研究提出了一种简便的靶标蛋白电子检测与定量方法，该方法整合了新型生物分子工程技术、便携式读数设备以及一次性纳米孔检测试纸条。我们设计的检测试剂可通过更换结合结构域来切换靶标对象：该试剂可在本体相中高效结合靶标，当靶标-试剂复合物通过纳米孔时，会产生独特的信号特征。本研究验证了检测试剂的模块化特性，分别以两种HIV抗体、肿瘤坏死因子α（TNFα）以及破伤风毒素作为靶标开展了验证实验。研究实现了从唾液拭子采样到出具结果的全流程检测，可在60秒内完成临床相关浓度范围（0.4~20 mg/L）的HIV抗体定量检测。尽管现有试纸类检测均为定性检测，但本方法可实现定量检测，同时为在单一便携式平台上同时实现免疫测定与分子诊断功能奠定了基础。