Transcriptional and post-transcriptional control of odorant receptor choice in ants

We generated snRNA-Seq for olfactory sensory neurons from late stage pupae of the clonal raider ant to study the chemosensory gene expression during development. Overall design: Antennal clubs of pupae were dissected and single nuclei extraction was done using a custom protocol for insect tissues. Library preparation was done using 10X Genomics 3' v3.1 kit. Sequencning was dine using Illumina NovaSeq S2 flowcell.

我们针对克隆掠夺蚁（clonal raider ant）蛹晚期的嗅觉感觉神经元开展了单细胞核RNA测序（snRNA-Seq），以研究其发育过程中的化学感应基因表达情况。整体实验设计：解剖蛹的触角棒，使用针对昆虫组织的定制实验方案提取单细胞核；文库制备采用10X Genomics 3'端v3.1试剂盒完成；测序使用Illumina NovaSeq S2测序流动槽完成。