Cooperation of estrogen and oocytes on gene expression in mouse cumulus cells

Oocyte-derived paracrine factors and estrogens cooperate to regulate the function and development of mouse cumulus cells. Cumulus oocyte complexes (COCs) were isolated from ovaries of mice. Oocytes were removed from some complexes (OOX). Groups were cultured with/without oocytes with/without estrogen and then cumulus cell transcriptome analyzed by microarrays.

卵母细胞来源的旁分泌因子（oocyte-derived paracrine factors）与雌激素（estrogens）协同调控小鼠卵丘细胞的功能与发育。从小鼠卵巢中分离得到卵丘卵母细胞复合体（cumulus oocyte complexes，COCs）。从部分复合体中移除卵母细胞（OOX）。将各组分别在有/无卵母细胞、有/无雌激素的条件下进行培养，随后通过基因芯片（microarrays）分析卵丘细胞的转录组。