High-throughput sequencing of small RNAs from Xenopus tropicalis

High-throughput sequencing of small RNAs from Xenopus tropicalis (adult liver, adult skin, oocytes stage I, II, III, IV, V, VI). total RNA, ~18-42 nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Overall design: Illumina/Solexa sequencing of adult liver, adult skin, oocytes stage I, II, III, IV, V, VI

本数据集为热带爪蟾（Xenopus tropicalis）来源的小分子RNA高通量测序数据，样本包括成年肝脏、成年皮肤以及I至VI期卵母细胞。实验流程为：提取总RNA后，通过聚丙烯酰胺凝胶电泳（PAGE）分离得到长度约18~42 nt的RNA片段；接头连接反应要求RNA片段带有5'单磷酸基团与3'羟基。整体实验设计：对上述成年肝脏、成年皮肤及I至VI期卵母细胞样本进行Illumina/Solexa高通量测序。