3D dataset of root soil bacteria dynamics obtained using large field of view light sheet microscope

A tailor made dual-illumination light-sheet system acquired photons scattered from the plant whilst fluorescence emissions were simultaneously captured from transparent soil particles and labelled microorganisms, allowing the generation of quantitative data on samples approximately 3600 mm³ in size with as good as 5 µm resolution at a rate of up to one scan every 30 minutes. The dataset shows the dynamics of Bacillus subtilis populations in the rhizosphere of lettuce plants in real time.本数据集依托定制化双照明光片成像系统（dual-illumination light-sheet system）生成，该系统可采集植物散射的光子，并同步捕获透明土壤颗粒与标记微生物的荧光发射信号，能够生成体积约3600立方毫米的样本的定量数据，成像分辨率可达5微米，扫描速率最高可达每30分钟一次。本数据集实时展示了莴苣根际枯草芽孢杆菌（Bacillus subtilis）种群的动态变化过程。