Intracellular pH measurements in the Pacific oyster (Crassostrea gigas)

Intracellular pH was measured using the dye BCECF (live cell imaging) in the mantle epithelial cells of the Pacific oyster (C. gigas). Intracellular pH was measured under the presence of modified seawater solutions (low sodium, low bicarbonate) and specific inhibitors for acid-base proteins were used. Value are presented as means.

本研究采用荧光染料BCECF结合活细胞成像技术，对太平洋牡蛎（C. gigas）外套膜上皮细胞的细胞内pH进行了检测。实验在低钠、低碳酸氢根的改性海水溶液条件下开展检测，并使用了酸碱蛋白特异性抑制剂。所得实验数据以平均值形式呈现。