Development of miRNA expression signatures for induction of histone deacetylase inhibitor (part 2)

To clarify the relationship between histone acetylation and miRNA expression, we performed miRNA arrays to detect histone deacetylase inhibitor (OBP-801) induced miRNA regulating AR expression in 22Rv1. Agilent-031181 Unrestricted Human miRNA Microarray (Agilent Technologies, Santa Clara, California) was used according to the manufacturer’s protocols.

为阐明组蛋白乙酰化（histone acetylation）与微小RNA（miRNA）表达之间的关联，本研究开展了微小RNA芯片实验，以检测经组蛋白去乙酰化酶抑制剂（histone deacetylase inhibitor，OBP-801）诱导的、调控雄激素受体（AR，Androgen Receptor）表达的微小RNA在22Rv1细胞中的表达情况。本实验依照制造商提供的操作方案，采用安捷伦-031181全人类微小RNA微阵列（Agilent Technologies，加利福尼亚州圣克拉拉市）完成检测。