Whole-Genome Methylation Profile of Fibroblast-Derived Isogenic Induced Pluripotent Stem Cells

The paper reports data on measuring whole-genome DNA methylation using an Illumina 450K Methylation BeadChip platform for isogenic induced pluripotent stem cells (iPSCs) derived from fibroblasts of a healthy donor and a PARK-associated Parkinson's disease patient via integrating (lentiviral transfection) and non-integrating (Sendai virus infection) reprogramming. Data on whole-genome methylation of neurons generated by differentiation of the iPSCs were also presented. The iPSCs were reprogrammed using the four Yamanaka’s factors: OCT4 (POU5F1), SOX2, KLF4, and cMYC. The data evidence that there are no significant differences in whole-genome methylation of iPSCs produced by various methods. Bisulphite converted DNA from the 12 samples were hybridised to the Illumina HumanMethylation450 BeadChip

本论文报告了基于Illumina 450K甲基化微珠芯片（Illumina 450K Methylation BeadChip）平台的全基因组DNA甲基化检测数据，检测样本为分别源自健康供体与PARK相关帕金森病患者成纤维细胞的同基因诱导多能干细胞（induced pluripotent stem cells，iPSCs），这些细胞分别通过整合型（慢病毒转染）与非整合型（仙台病毒感染）重编程方法构建。本研究同时提供了上述诱导多能干细胞分化所得神经元的全基因组甲基化数据。该批诱导多能干细胞均采用山中伸弥四因子（OCT4(POU5F1)、SOX2、KLF4及cMYC）进行重编程。数据集数据证实，不同重编程方法制备的诱导多能干细胞的全基因组甲基化水平无显著差异。本研究对12个样本的亚硫酸氢盐转化DNA开展了Illumina HumanMethylation450 微珠芯片杂交检测。