Region-specific expressions of ten-selected <i>OPRM1</i> splice variants.

Each panel represents the regional expression of one variant in four inbred mouse strains. Red bar: B6 mice; Green bar: 129 mice; Blue bar: SJL mice; Brown bar: SWR mice. Bars represent the mean of E−ΔC(t) values ± S.E.M. Pfc: prefrontal cortex; Str: striatum; Tha: thalamus; Hyp: hypothalamus; Hip: hippocampus; PAG: periaqueductal gray; BS: brainstem; Cb: cerebellum; Spc: spinal cord; WB: whole brain. mE1-2 represents all full-length 7-TM variants. Significant difference was calculated by Two-way ANOVA with Tukey’s multiple comparisons test for each variant (Prism 5.0). The results of the statistical analysis were listed in Table S3. The expression profiles of the other 19 OPRM1 splice variants are shown in Figures S1 & S2.每个面板展示四种近交系小鼠品系中某一剪接变体的脑区表达模式。柱形颜色对应如下：红色柱形代表B6小鼠，绿色柱形代表129小鼠，蓝色柱形代表SJL小鼠，棕色柱形代表SWR小鼠。柱形高度表示E−ΔC(t)值的平均值±标准误（S.E.M.）。各缩写对应的脑区为：Pfc（前额叶皮层，prefrontal cortex）、Str（纹状体，striatum）、Tha（丘脑，thalamus）、Hyp（下丘脑，hypothalamus）、Hip（海马体，hippocampus）、PAG（中脑导水管周围灰质，periaqueductal gray）、BS（脑干，brainstem）、Cb（小脑，cerebellum）、Spc（脊髓，spinal cord）、WB（全脑，whole brain）。mE1-2代表所有全长七次跨膜（7-TM）变体。针对每个变体的显著性差异通过双因素方差分析（Two-way ANOVA）结合Tukey多重比较检验进行计算，所用分析软件为Prism 5.0。该统计分析结果详见补充表S3。其余19种OPRM1剪接变体的表达谱详见补充图S1与S2。