Chip-seq analysis of meiotic Cohesin (mei-Cohesins) subunit REC8 cleaved by separase (REC8*), and SMC3 in Macaca fascicularis testis

The study aimed to map epigenomic locations of separase-cleaved REC8 using chromatin IP analyzed by high-throughput sequencing on purified germ cells from M. fascicularis testis M. fascicularis is a monkey with non-seasonal male germ cells production, and the most comon primate used for preclinical animal studies. The ChIP-on-CHEP study was done with proprienaly mAbs developed against the COOH-terminal peptides of REC8* after cleavage by separase: X-LLEIPR* and X-EIEVPR*

本研究旨在通过经高通量测序（high-throughput sequencing）分析的染色质免疫沉淀（chromatin IP）技术，对食蟹猕猴（M. fascicularis）睾丸纯化生殖细胞中经分离酶（separase）切割的REC8蛋白的表观基因组位点进行定位绘制。食蟹猕猴是一类雄性生殖细胞生成无季节性的非人灵长类动物，同时也是临床前动物研究中最常用的灵长类实验动物。本次ChIP-on-CHEP实验采用了针对分离酶切割后REC8蛋白羧基末端肽段X-LLEIPR*与X-EIEVPR*开发的专有单克隆抗体（mAbs）完成。