Deep phenotyping the right ventricle to establish translational MRI biomarkers for characterization of adaptive and maladaptive states in pulmonary hypertension

We performed molecular phenotyping of RV remodelling in pulmonary hypertension (PH), using transcriptome analysis of RV tissue obtained from SuHx-induced male rats.When compared to human RV decompensated PH, the molecular characterisation of these rats showed that ECM and collagen fibrillar organisation were highly upregulated in SuHx rats and human profiles along with responses to cytokines. Moreover, growth differential factor was found common in SuHx and RV human samples. Overall design: RNA sequencing was performed on rat RV tissues which were classfied as maladaptive SuHx (n=3) as well as healthy RV from control rats (n=3).

我们针对肺动脉高压（pulmonary hypertension, PH）中的右心室（right ventricle, RV）重塑开展分子表型分析，采用从SuHx诱导的雄性大鼠体内获取的右心室组织进行转录组分析。
将该大鼠模型与人类失代偿性肺动脉高压的右心室样本进行对比后，分子特征分析结果显示：SuHx模型大鼠与人类样本中，细胞外基质（extracellular matrix, ECM）及胶原纤维组织均显著上调，同时伴随细胞因子应答反应。
此外，生长分化因子（growth differentiation factor）在SuHx模型大鼠与人类右心室样本中均存在共同表达。
实验整体设计：对两组大鼠的右心室组织开展RNA测序，其中适应性不良SuHx模型组（n=3），以及对照组健康大鼠的右心室组织（n=3）。