Comparison of apical leaf halves of Arabidopsis thaliana PP2A-B´-mutants and wild type plants

The objective of this RNAseq-experiment has been to compare apical leaf halves of different mutants with that of wild type plants. Overall design: mRNA profiles of 5 pools each of apical and basal leaf halves of seven-week-old Arabidopsis thaliana wild type (Col-0) plants and mutant pp2a-b'?, double mutants pp2a-b'? sid2 and pp2a-b'?? and complementation line pp2a-b´? P35S:PP2A-B'? were generated by sequencing using Illumina HiSeq 3000.本RNA测序（RNAseq）实验旨在对比不同突变体与野生型植株的叶尖半片组织。实验整体设计为：对七周龄拟南芥（Arabidopsis thaliana）野生型（Col-0）、单突变体pp2a-b'?、双突变体pp2a-b'? sid2与pp2a-b'??，以及互补株系pp2a-b´? P35S:PP2A-B'?的叶尖与叶基半片组织各制备5个混合样本，采用Illumina HiSeq 3000进行测序，以获取其mRNA表达谱。