BM-resident BL/6 and ST2-/- ILC2s cultured with or without rmIL-33

To investigate the effect of interleukin (IL-33) on bone marrow (BM) resident type 2 innate lymphoid cells (ILC2s), we treated BL/6 and ST2-knockout (ST2-/-) ILC2s for 24h with recombinant mouse IL-33 We then performed gene expression profiling analysis using data obtained from RNA-seq of 3-4 samples per condition. Comparative gene expression profiling analysis of RNA-seq data for FACS-sorted BL/6 ILC2s without IL-33 treatment, BL/6 ILC2s with IL-33 treatment, and ST2-/- ILC2s without IL-33 treatment.

为探究白细胞介素33（interleukin-33, IL-33）对骨髓（bone marrow, BM）驻留型2型先天淋巴细胞（type 2 innate lymphoid cells, ILC2s）的调控效应，本研究采用重组小鼠IL-33分别处理BL/6小鼠来源及ST2基因敲除（ST2-knockout, ST2-/-）的ILC2细胞，处理时长为24小时。随后针对每一组实验条件的3-4份样本，通过RNA测序（RNA-seq）获取转录组数据并开展基因表达谱分析。本研究对三类经荧光激活细胞分选（fluorescence-activated cell sorting, FACS）纯化的样本的RNA测序数据进行了比较基因表达谱分析：未施加IL-33处理的BL/6 ILC2、经IL-33处理的BL/6 ILC2，以及未施加IL-33处理的ST2-/- ILC2。