miRNA Expression data from synovium in mouse serum transfer arthritis model (STA) model

To find regulated miRNAs during peak inflammation of rheumatoid arthritis (RA), we have collected synovium from mouse STA model at day 0 (Non Arthritic) and day 10 (Peak Inflammation). For miRNA profiling, we used high-throughput BioMark Real-Time PCR system (Fluidigm, South San Francisco, CA) We analyzed expression of 750 miRNA in Non-arthritic synovium pooled from 3 mice and Arthritic synovium at peak inflammation pooled from 10 mice. Two biological replicates for each time points. Two technical replicates per sample.

为筛选类风湿关节炎（RA，rheumatoid arthritis）炎症峰值阶段的调控性微小核糖核酸（miRNA，microRNA），我们采集了小鼠STA模型在第0天（非关节炎状态）和第10天（炎症峰值阶段）的滑膜组织。为开展miRNA表达谱分析，我们采用了高通量BioMark实时荧光定量PCR系统（Fluidigm公司，美国加利福尼亚州南旧金山）。我们分别对3只小鼠混合的非关节炎滑膜组织，以及10只小鼠混合的炎症峰值阶段关节炎滑膜组织中的750种miRNA表达水平进行了分析。每个时间点设置2次生物学重复，每份样本设置2次技术重复。