Differential miRNA expression profiles of human vascular endothelial cells (VECs) between Type-I pro-proliferative/pro-stenotic VECs and Type-II anti-proliferative/anti-stenotic VECs [Agilent-046064]. Homo sapiens

Human VECs are categorized into two groups according to their effects on the proliferation of vascular smooth muscle cells (in vitro) and the induction of stenosis in endothelia-removed arteries after transplantation (In vivo): pro-proliferative/pro-stenotic (Type-I) virsus anti-proliferative/anti-stenotic (Type-II) VECs. Since RGS5, which is a master gene responsible for aging- and oxidative stress-dependent Type-II to Type-I conversion, is the only protein-coding gene that shows differential expression profiles between Type-I and Type-II VECs, non-coding RNAs including miRNA should be working at the downstream of RGS5 for quality control of VECs. Overall design: Totally 13 samples of Type-I VECs (HRMVEC, HCAEC, HMVEC, HBVEC, aged ES(KhES1)dEC, aged ES(KhES3)dEC) and Type-II VECs (young ES(KhES1)dEC, young ES(KhES3)dECs, SeViPS(BJ)dEC_lot1, SeViPS(BJ)dEC_lot2, SeViPS(HUVEC)dEC_lot1, SeViPS(HUVEC)dEC_lot2, SeViPS(HUVEC)dEC_lot3) ( (Nishio et al., World J Transl Med 4: 88-100. DOI:10.5528/wjtm.v4.i3.101) were subjected to the analyses.

人类血管内皮细胞（Vascular Endothelial Cells，以下简称VECs）可根据其对血管平滑肌细胞增殖的影响（体外实验）以及移植后去内皮动脉的狭窄诱导效应（体内实验）分为两类：促增殖/致狭窄的I型（Type-I）VECs，与抗增殖/抗狭窄的II型（Type-II）VECs。鉴于RGS5作为介导衰老及氧化应激依赖的II型向I型VECs转化的关键主效基因，是唯一在I型与II型VECs间呈现差异表达谱的蛋白编码基因，因此包括微小RNA（microRNA，miRNA）在内的非编码RNA应作用于RGS5下游，以实现VECs的表型质控。整体实验设计：共纳入13例样本，其中I型VECs样本包括HRMVEC、HCAEC、HMVEC、HBVEC、衰老型ES(KhES1)dEC、衰老型ES(KhES3)dEC；II型VECs样本包括年轻型ES(KhES1)dEC、年轻型ES(KhES3)dECs、SeViPS(BJ)dEC批次1、SeViPS(BJ)dEC批次2、SeViPS(HUVEC)dEC批次1、SeViPS(HUVEC)dEC批次2、SeViPS(HUVEC)dEC批次3（引用来源：Nishio等, World J Transl Med 4: 88-100, DOI:10.5528/wjtm.v4.i3.101），所有样本均用于本次分析。