LKB1 orchestrates dendritic cell metabolic quiescence and anti-tumor immunity [HT_MG-430_PM]. LKB1 orchestrates dendritic cell metabolic quiescence and anti-tumor immunity [HT_MG-430_PM]

Loss of LKB1 leads to aberrant DC maturation and production of cytokines and immunoregulatory molecules. DCs (n = 7 per genotype) were isolated from the spleen, and CD25+CD4+ Tregs (n = 4 for WT, n = 3 for LKB1ΔDC mice) were isolated from the spleen and peripheral lymph nodes of WT and LKB1ΔDC mice. Overall design: This series includes 6 samples of splenic DCs (3 WT and 3 LKB1 KO DCs)

LKB1基因缺失会导致树突状细胞（Dendritic Cells, DC）成熟异常，并影响细胞因子与免疫调节分子的产生。研究人员从两种基因型小鼠的脾脏中分离树突状细胞（每组样本量n=7）；同时从野生型（Wild Type, WT）及LKB1ΔDC小鼠的脾脏与外周淋巴结中分离CD25+CD4+调节性T细胞（Regulatory T cells, Tregs：野生型组n=4，LKB1ΔDC小鼠组n=3）。实验整体设计：本数据集包含6份脾脏树突状细胞样本，其中野生型组与LKB1敲除（Knockout, KO）组各3份。