Umbilical cord miRNA profile associated with catch-up growth in SGA infants

The aim of this study was to identify umbilical cord microRNA (miRNA) associated with catch-up growth in SGA infants. miRCURY LNA™ Universal RT microRNA PCR Human Panel I+II (Exiqon) were used to study the miRNA profile in umbilical cord tissue of 5 SGA infants with catch-up (SGA-CU), 5 SGA infants without catch-up (SGA-nonCU) and 5 control infants (appropriate-for-gestational-age, AGA). Catch-up differentially expressed miRNA were studied and validated in a larger cohort. qPCR gene expression profiling in umbilical cord from 15 subjects (5 SGA-CU, 5 SGA-nonCU and 5 AGA).

本研究旨在筛选与小于胎龄儿（small for gestational age, SGA）追赶生长相关的脐带微RNA（microRNA, miRNA）。本研究使用miRCURY LNA™ Universal RT微RNA PCR人类组合试剂盒I+II（艾克森，Exiqon），对5例追赶生长型小于胎龄儿（SGA-CU）、5例非追赶生长型小于胎龄儿（SGA-nonCU）及5例适于胎龄儿（appropriate-for-gestational-age, AGA）对照婴儿的脐带组织miRNA表达谱进行检测。针对筛选出的与追赶生长相关的差异表达miRNA，本研究在更大规模队列中完成验证，并通过实时定量聚合酶链反应（qPCR）对上述15例受试者（5例SGA-CU、5例SGA-nonCU及5例AGA）的脐带组织开展基因表达谱分析。