Diesel exhaust and house dust mite allergen lead to common changes in the airway methylome and hydroxymethylome. Diesel exhaust and house dust mite allergen lead to common changes in the airway methylome and hydroxymethylome

Genomewide DNA methylation profiling of saline, diesel exhaust particiles and house dust mite treated human airway epithelial cells. Methylation profiles were generated by the Illumina Infinium MethylationEPIC beadchips. Overall design: Human bronchial epithelial cells were treated with PBS, diesel exhaust particles (5ug/cm2), and house dust mite (25ug/ml) for 24 hours. Genomic DNA were extracted and treated with or without oxidation following the TrueMethyl® protocol (NuGen) followed by bisulifte conversion before being hybridized to the Illumina Infinium MethylationEPIC beadchips

本数据集针对生理盐水、柴油机尾气颗粒物及屋尘螨处理的人气道上皮细胞开展全基因组DNA甲基化谱分析。甲基化谱通过Illumina Infinium MethylationEPIC 微珠芯片（Illumina Infinium MethylationEPIC BeadChip）生成。实验设计概况：将人支气管上皮细胞分别以磷酸盐缓冲液（Phosphate Buffered Saline，PBS）、柴油机尾气颗粒物（浓度为5μg/cm²）及屋尘螨（浓度为25μg/ml）处理24小时。提取基因组DNA后，按照TrueMethyl®试剂盒（NuGen公司）的操作流程进行氧化与非氧化处理，随后进行亚硫酸氢盐转化，最后将样本与Illumina Infinium MethylationEPIC 微珠芯片进行杂交。