Sequence of the UbeK trypanosomal degron
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Sequence of the UbeK degron signal used to decrease the half life of GFP expressed in Trypanosoma brucei brucei (T. b. brucei). The UbeK degron consists of 76 residues of native T. b. brucei ubiquitin protein, at the N-terminus and ending in a leucine residue to signal proteasomal degradation, followed by a short ‘eK’ region which featuring an additional proteasomal degradation signal of two lysine residues flanking an arginine.
本数据集为用于缩短布氏布氏锥虫(Trypanosoma brucei brucei,T. b. brucei)中表达的绿色荧光蛋白(Green Fluorescent Protein,GFP)半衰期的UbeK降解信号(UbeK degron)序列。UbeK降解信号由76个天然布氏布氏锥虫泛素蛋白的氨基酸残基组成,位于多肽链的N端(氨基末端),且以亮氨酸残基结尾以作为蛋白酶体降解的信号;其后衔接一段短的"eK"区域,该区域包含一个额外的蛋白酶体降解信号:由两个赖氨酸残基侧翼环绕一个精氨酸残基构成。
创建时间:
2021-06-25




