Genome-wide analysis of SRC-1/NCOA1 and Erα/ESR1 binding in chromatin obtained from the ERα stably transfected U2OS osteosarcoma cell line (U2OS-ERα) with ChIP-SEQ.

To better understand the role of SRC-1 in estrogen signaling in bone, a genome-wide analysis of ERα and SRC-1 binding sites in the absense and presence of E2 was conducted. Cells were grown in CSS media for 3 days, and treated with vehicle (EtOH) or estrogen (10^-8M) for 45 minutes and immediately harvested for chomatin immunoprecipitation (ChIP)

为深入解析类固醇受体共激活因子1（SRC-1）在骨骼雌激素信号通路中的功能，本研究针对雌二醇（E2）存在与缺失两种条件下的雌激素受体α（ERα）与SRC-1结合位点开展了全基因组分析。实验细胞于活性炭剥离血清（charcoal-stripped serum, CSS）培养基中培养3天，随后分别以溶剂对照（乙醇，EtOH）或10^-8 M雌激素处理45分钟，即刻收集样本用于染色质免疫沉淀（chromatin immunoprecipitation, ChIP）实验。