Differential gene expression profiles are dependent upon method of peripheral blood RNA isolation (direct, heparin)

RNA isolation and purification steps greatly influence the results of gene expression profiling. There are two commercially available products for whole blood RNA collection, PAXgene and Tempus blood collection tubes, and each comes with their own RNA purification method. We examined the impact of RNA isolation methods on gene expression profiles. We demonstrated that peripheral blood RNA isoaltion can critically impact differential expression results, particularly in the clinical setting where fold-change differences are typically small and there is inherent variability within biological corhorts Keywords: micorarray quality assurance Comparison of 5 healthy control samples drawn directly in PAXgene or Tempus tubes vs. the same 5 healthy control samples drawn in Li Heparin tubes with no PHA stimulation.

RNA分离与纯化步骤对基因表达谱分析结果具有显著影响。目前市售的全血RNA采集产品共有两款：PAXgene采血管与Tempus采血管，二者各自配套专属的RNA纯化方案。本研究考察了RNA分离方法对基因表达谱的影响，证实外周血RNA分离方式会对差异表达分析结果产生关键性影响，尤其在临床场景中——此类场景下差异倍数通常较小，且生物队列本身存在固有变异。关键词：微阵列质量保证；直接采用PAXgene或Tempus采血管采集的5份健康对照样本，与同5份采用肝素锂采血管采集且未经过PHA刺激的健康对照样本的比较。