Compared the gene expression profiles between the PTL-Her2-CAR-T cells and Her2-CAR-T cells isolated from tumor tissues. Compared the gene expression profiles between the PTL-Her2-CAR-T cells and Her2-CAR-T cells isolated from tumor tissues

To further development of different gene expression between the PTL-Her2-CAR-T cells (knockdown of PD-1, Tim-3, Lag-3 by shRNA-clusters in Her2-CAR-T cells) and Her2-CAR-T cells, we have employed whole genome microarray expression profiling as a discovery platform. Overall design: SKOV3-bearing NSG mice were injected with various CAR-T cells via tail. At 28 days after CAR-T-cells infusion, mice were euthanized and tumor tissues were resected. The tumor was divided and tumor infiltrating CAR-T cells were separated for RNA-seq.

为深入阐明PTL-Her2嵌合抗原受体T细胞（PTL-Her2-CAR-T cells，即通过短发夹RNA (short hairpin RNA, shRNA) 簇在靶向Her2的嵌合抗原受体T细胞 (Chimeric Antigen Receptor T-Cell, CAR-T) 中敲低程序性死亡受体1 (Programmed Death 1, PD-1)、T细胞免疫球蛋白黏蛋白3 (T-cell Immunoglobulin and Mucin domain-containing protein 3, Tim-3) 及淋巴细胞活化基因3 (Lymphocyte Activation Gene 3, Lag-3) 的工程化细胞）与靶向Her2的CAR-T细胞之间的基因表达差异，本研究采用全基因组微阵列表达谱分析作为初步发现筛选平台。
实验整体设计：将携带SKOV3肿瘤细胞的NSG免疫缺陷小鼠经尾静脉注射不同CAR-T细胞；在CAR-T细胞输注后第28天处死小鼠并切除肿瘤组织，将肿瘤组织解离后分离其中的肿瘤浸润性CAR-T细胞，用于RNA测序 (RNA-seq)。