Regulation of mesenchymal stem to transit amplifying cell transition in the continuously growing mouse incisor [Histone ChIP]

Regulation of transit amplifying cell formation from self-renewing stem cell is fundamental process for cell replacement in a controlled way. Here we analyse the properties of a population of mesenchymal TACs in the continuously growing mouse incisor to identify key components of the molecular regulation that drives proliferation. We show that the polycomb repressive complex 1 acts as a global regulator of the TAC phenotype by its direct action on the expression of key cell cycle regulatory genes and also by regulating Wnt/b-catenin signalling activity. We also identify an essential requirement for TACs in maintaining the mesenchymal stem cells, indicative of a positive feedback mechanism. Analysing the properties of mesenchymal transit amplifying cells population and identifing key components of the molecular regulation that drives proliferation. Overall design: Histone ChIPseq without input sample (SICER algorithm used for analysis does not reqire input sample)从自我更新干细胞生成转运扩增细胞（transit amplifying cell，以下简称TAC）的调控过程，是实现可控细胞替换的核心生物学机制。本研究以持续生长的小鼠切牙中的间充质TAC群体为研究对象，通过分析其特性以鉴定驱动细胞增殖的分子调控关键组分。研究证实，多梳抑制复合体1（polycomb repressive complex 1）可通过直接调控关键细胞周期调控基因的表达，以及调节Wnt/β-连环蛋白信号通路活性，作为TAC表型的全局调控因子。本研究同时发现，TAC对于维持间充质干细胞具有不可或缺的作用，这提示存在正向反馈调控机制。本研究针对间充质转运扩增细胞群体的特性展开分析，并鉴定驱动细胞增殖的分子调控关键组分。实验整体设计：采用无输入样本的组蛋白Histone ChIPseq测序分析，本次分析所使用的SICER算法无需输入样本。