Information of the 34 OUTs from each sample.

Microbes are an important part of the vineyard ecosystem, which significantly influence the quality of grapes. Previously, we identified a bud mutant variety (named ‘Fengzao’) from ‘Kyoho’ grapes. The variation of microbial communities in grape and its bud mutant variety has not been studied yet. So, in this study, with the samples of both ‘Fengzao’ and ‘Kyoho’, we conducted high-throughput microbiome sequencing and investigated their microbial communities in different tissues. Obvious differences were observed in the microbial communities between ‘Fengzao’ and ‘Kyoho’. The fruit and the stem are the tissues with relatively higher abundance of microbes, while the leaves contained less microbes. The fruit and the stem of ‘Kyoho’ and the stem of ‘Fengzao’ had relatively higher species diversity based on the alpha diversity analysis. Proteobacteria, Enterobacteriaceae and Rhodobacteraceae had significantly high abundance in ‘Fengzao’. Firmicutes and Pseudomonas were highly abundant in the stems of ‘Kyoho’, and family of Spirochaetaceae, Anaplasmataceae, Chlorobiaceae, and Sphingomonadaceae, and genera of Spirochaeta, Sphingomonas, Chlorobaculum and Wolbachia were abundant in the fruits of ‘Kyoho’. These identified microbes are main components of the microbial communities, and could be important regulators of grapevine growth and development. This study revealed the differences in the microbial compositions between ‘Kyoho’ and its bud mutant, and these identified microbes will be significant resources for the future researches on the quality regulation and disease control of grapevines.微生物是葡萄园生态系统的重要组成部分，对葡萄果实品质具有显著影响。此前，我们从‘巨峰（Kyoho）’葡萄中鉴定出一株芽变品种，命名为‘丰早（Fengzao）’。目前尚未针对葡萄及其芽变品种的微生物群落变异开展相关研究。因此，本研究以‘丰早’与‘巨峰’的不同组织样本为材料，开展高通量微生物组测序，分析二者不同组织的微生物群落组成。结果显示，‘丰早’与‘巨峰’的微生物群落组成存在显著差异。葡萄果实与茎秆的微生物丰度相对较高，叶片微生物丰度则较低。通过α多样性分析可知，巨峰的果实、茎秆以及丰早的茎秆具有相对更高的物种多样性。变形菌门(Proteobacteria)、肠杆菌科(Enterobacteriaceae)与红杆菌科(Rhodobacteraceae)在‘丰早’中显著富集；厚壁菌门(Firmicutes)和假单胞菌属(Pseudomonas)在‘巨峰’的茎秆中高度富集；而螺旋体科(Spirochaetaceae)、无形体科(Anaplasmataceae)、绿菌科(Chlorobiaceae)与鞘氨醇单胞菌科(Sphingomonadaceae)，以及螺旋体属(Spirochaeta)、鞘氨醇单胞菌属(Sphingomonas)、绿杆菌属(Chlorobaculum)和沃尔巴克氏体属(Wolbachia)在‘巨峰’的果实中大量存在。上述鉴定得到的微生物是葡萄微生物群落的核心组成部分，可能在葡萄生长发育过程中发挥重要调控作用。本研究揭示了巨峰与其芽变品种间的微生物群落组成差异，所鉴定得到的微生物将为未来葡萄品质调控与病害防控研究提供重要的研究资源。