Optimizing reprogramming from a range of blood volumes across multiple donors.

The table represents reprogramming efficiencies following optimization trials that began with a set of donors providing different volumes of blood. “iPSCs per ml blood” represents the number of total iPSCs as determined by their ability to stain positively for Tra1-81 expression and exhibit characteristic, ES-like morphology divided by the volume of blood processed from the donor. “iPSCs per 1×105” cells represents the number of total iPSCs divided by the total number of cells used for each transfection after 6 days of expansion multiplied by 1×105 cells. Set 1 refers to plasmid combination Set 1 expressing C-myc and Set 2 refers to the triple plasmid combination set using expressing L-myc. Asterisks indicate the generation of iPSCs from CD34+ cells isolated fresh from blood while all other trials were performed from CD34+ cells isolated from PBMCs that were frozen down immediately after isolation from blood. N indicates the number of independent reprogramming trials performed for each donor. Reprogramming efficiencies were averaged where N>1.