Optimizing reprogramming from a range of blood volumes across multiple donors.

The table represents reprogramming efficiencies following optimization trials that began with a set of donors providing different volumes of blood. “iPSCs per ml blood” represents the number of total iPSCs as determined by their ability to stain positively for Tra1-81 expression and exhibit characteristic, ES-like morphology divided by the volume of blood processed from the donor. “iPSCs per 1×105” cells represents the number of total iPSCs divided by the total number of cells used for each transfection after 6 days of expansion multiplied by 1×105 cells. Set 1 refers to plasmid combination Set 1 expressing C-myc and Set 2 refers to the triple plasmid combination set using expressing L-myc. Asterisks indicate the generation of iPSCs from CD34+ cells isolated fresh from blood while all other trials were performed from CD34+ cells isolated from PBMCs that were frozen down immediately after isolation from blood. N indicates the number of independent reprogramming trials performed for each donor. Reprogramming efficiencies were averaged where N>1.本表格展示了以不同采血体积的供体为起始对象的优化重编程试验所得到的重编程效率。“每毫升血液诱导多能干细胞（induced pluripotent stem cells, iPSCs）”指标为：经Tra1-81表达阳性染色且呈现典型胚胎干细胞（Embryonic Stem Cells, ES）样特征形态的总诱导多能干细胞数量，除以供体血液处理体积后所得的数值。“每1×10^5个细胞诱导多能干细胞”指标为：扩增6天后，总诱导多能干细胞数除以单次转染所用的总细胞数，再乘以1×10^5个细胞所得的数值。组合1为表达C-myc的质粒组合1，组合2为表达L-myc的三质粒组合体系。星号标注代表从新鲜分离的血液中获取的CD34阳性（CD34+）细胞中重编程得到诱导多能干细胞，其余所有试验均采用从血液分离后立即冻存的外周血单个核细胞（Peripheral Blood Mononuclear Cells, PBMC）中分离得到的CD34阳性细胞开展。N代表每位供体开展的独立重编程试验次数；当N>1时，重编程效率取平均值。