Wheat DAP-seq for NAC5, NAM1 and NAM2

The aim of this study was to identify the downstream target genes of three wheat NAC transcription factors associated with senescence. The genes studied were NAC5, NAM1 and NAM2 with 2 or 3 homoeologs for each gene. DNA affinity purification sequencing (DAP-seq) was used, with the DNA library constructed from wheat flag leaves cv Cadenza 7 and 14 days after heading.本研究旨在鉴定3个与衰老相关的小麦NAC转录因子（NAC transcription factors）的下游靶基因。本研究所涉及的基因为NAC5、NAM1和NAM2，每个基因拥有2或3个部分同源基因。本研究采用DNA亲和纯化测序（DNA affinity purification sequencing，DAP-seq）技术，以小麦品种Cadenza抽穗后7天和14天的旗叶为材料构建DNA文库。