Cynomolgus macaques treated with Divalent-siRNA (Di-siRNA) targeting Huntingtin gene

Examine potential off-target effects of HTT targeting siRNA in cynomologus macaque brains. Overall design: RNA-seq of brain punches from HTT targeting siRNA treated and non-treated cynomolgus macaques (N=4 per group). Three brain punches were taken from each animal and processed/sequenced as separate samples. Reads were processed and combined for analysis.本数据集旨在探究靶向HTT的小干扰RNA（small interfering RNA, siRNA）在食蟹猴（cynomolgus macaque）大脑中的潜在脱靶效应。 总体实验设计：对经HTT靶向siRNA处理组与未处理组的食蟹猴（每组n=4）的脑组织穿刺样本进行RNA测序（RNA-seq）。每只动物采集3份脑组织穿刺样本，作为独立样本分别进行处理与测序；后续对测序读段进行预处理后合并，用于数据分析。