Endoderm Differentiation - Single Cell RNA-sequencing

Single-cell RNA-seq (scRNA-seq) on nocodazole and DMSO treated cells before and after differentiation into endoderm. hPSC colonies were treated with DMSO or 100ng/ml nocodazole for 16 hours and induced to differentiate into definitive endoderm for three days. Single cells were subsequently collected in either undifferentiated conditions (Und) or after 3 days of endoderm differentiation (Endoderm) and then sorted onto 384 well plates for Smart-Seq2 processing.

本数据集针对经诺考达唑与二甲基亚砜（DMSO）处理的、向内胚层分化前后的细胞开展单细胞RNA测序（scRNA-seq）。具体实验流程如下：将人类多能干细胞（hPSC）集落以二甲基亚砜（DMSO）或100ng/ml诺考达唑处理16小时，随后诱导其向定形内胚层分化3天；随后分别采集未分化状态（Und）以及内胚层分化3天后（Endoderm）的单细胞，将其分选至384孔板中，用于Smart-Seq2测序流程。