H3K27ac ChIP sequencing of low and high pigment B16 cells [B16_D5_LP_HP_ChIPSeq]

In one of our earlier studies, we have shown that, through a pH-mediated feed-forward loop, H3K27ac selectively activates pigmentation genes and accentuates melanocyte differentiation at the population level. Here we used the B16 progressive pigmentaiton model that harbours multiple melanocyte states in the same culture. We sorted the low and high pigment cells at day 5 and investigated the differences in H3K27ac mark between the low and high pigment melanocyte sub-populations. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modification H3K27ac in low and high pigment B16 cells sorted from the same culture.

在我们此前的一项研究中，我们已证实，通过pH介导的前馈环路（pH-mediated feed-forward loop），组蛋白H3第27位赖氨酸乙酰化（H3K27ac）可选择性激活色素沉着相关基因，并在群体水平上增强黑素细胞分化。本研究采用了B16进行性色素沉着模型，该模型可在同一培养体系中呈现多种黑素细胞状态。我们在第5日分选了低色素与高色素细胞，并探究了低、高色素黑素细胞亚群之间的H3K27ac标记差异。实验整体设计：对从同一培养体系中分选得到的低、高色素B16细胞中的组蛋白修饰H3K27ac进行染色质免疫共沉淀测序（ChIP-seq）。