Primers used for qPCR to detect the heat tolerance-related SNP all-53308-760 T/C.

*The bases bold are an allele-specific base, and the bases in lowercase are a deliberate mismatch.**The amplification efficiency of the allele-specific forward primer was determined in combination with all-53308 reverse primer.

*加粗碱基为等位基因特异性碱基（allele-specific base），小写碱基为故意错配碱基。
*本研究将等位基因特异性正向引物（allele-specific forward primer）与all-53308反向引物组合使用，对其扩增效率进行了测定。