Total and nuclear RNA-seq of whole-embryo and mesodermal cells

Whole-transcriptome analysis using total RNA-seq (rRNA depleted) of Drosophila melanogaster samples from whole-embryo or mesodermal FACS-sorted cells (using a transgenic fly line expressing a EGFP-tagged protein in mesoderm, during different stages of early embryonic development.

本数据集针对黑腹果蝇（Drosophila melanogaster）的两类样本开展全转录组分析，采用总RNA测序（rRNA depleted，去除核糖体RNA）技术：一类为全胚胎样本，另一类为经荧光激活细胞分选（FACS）得到的中胚层细胞样本。其中，中胚层细胞取自可在早期胚胎发育不同阶段的中胚层中表达增强型绿色荧光蛋白（EGFP）标记蛋白的转基因果蝇品系。