Expression data from 4-week-old mouse liver

mRNA degradation critically contributes to liver development and function. The CCR4-NOT complex serves as a major deadenylase that initiates mRNA degradation. We used microarrays to identify deregulated genes in the livers lacking CNOT3, a core subunit of the CCR4-NOT complex. Liver-specific CNOT3 knockout mice were generated by expressing Cre recombinase under the control of Albumin promoter. Livers from control and CNOT3 knockout mice were used for RNA extraction and hybridization on Affymetrix microarrays.

mRNA降解对肝脏发育和功能起到关键作用。CCR4-NOT复合物是启动mRNA降解的主要脱腺苷酸酶（deadenylase）。我们通过微阵列（microarrays）技术，鉴定缺失CCR4-NOT复合物核心亚基CNOT3的肝脏组织中的失调基因。本研究通过在白蛋白启动子（Albumin promoter）调控下表达Cre重组酶（Cre recombinase），构建了肝脏特异性CNOT3敲除小鼠。随后，收集对照组与CNOT3敲除小鼠的肝脏组织，用于RNA提取及Affymetrix微阵列杂交实验。