Effects of siruin disruption on Aspergillus nidulans transcriptome

Sirtuin is a ubiquitous class III histone deacetylase (HDAC) that deacetylates acetyllysine residues of histones, and epigenetically regulates global transcription. We investigated a sirtuin isozyme (SirE, AN1226 encoding protein) of ascomycetes fungus Aspergillus nidulans. We used RNA-seq analyses for Aspergillus nidulans wild type strain and SirEd strain, a gene disruptant of sirE to elucidate the physiological roles of SirE.

沉默信息调节因子（Sirtuin）是一类遍在的III类组蛋白去乙酰化酶（HDAC），可对组蛋白的乙酰赖氨酸残基进行去乙酰化修饰，并通过表观遗传机制调控全局转录。我们针对子囊菌构巢曲霉（Aspergillus nidulans）中的一种沉默信息调节因子同工酶（SirE，即AN1226编码的蛋白）展开了研究。为阐明SirE的生理功能，我们采用RNA测序（RNA-seq）分析了构巢曲霉的野生型菌株与sirE基因敲除菌株SirEd。