Transcriptomic analysis from lateral ganglionic eminence (LGE) of wild type and Gsx2 germline knock-out mice.

RNA-seq analysis was used to identify genes that are differentially expressed between wild type and Gsx2 null mouse LGEs. Overall design: Bulk RNA-seq was performed on dissected E12.5 mouse LGE from wild type and Gsx2 null embryos in quadruplicate

本研究采用RNA测序（RNA-seq）分析，旨在鉴定野生型与Gsx2纯合缺失小鼠外侧神经节隆起（Lateral Ganglionic Eminence, LGE）之间的差异表达基因。实验设计概述：针对取自野生型及Gsx2纯合缺失胚胎的小鼠胚胎发育第12.5天外侧神经节隆起样本，开展批量RNA测序（Bulk RNA-seq），每组设置四个生物学重复。