KNP_vs_YS_Fat. Sus scrofa

Anim Genet. 2009 Feb;40(1):115-8. Epub 2008 Oct 17. Differentially expressed transcripts in adipose tissue between Korean native pig and Yorkshire breeds. Moon JK, Kim KS, Kim JJ, Choi BH, Cho BW, Kim TH, Lee CK. Division of Biotechnology, College of Life Sciences & Biotechnology, Korea University, Seoul, South Korea. We measured and compared the transcripts of adipose tissue from Korean native pig (KNP) and Yorkshire (YS) breeds to investigate breed-specific transcription changes. We employed both the Affymetrix porcine genome array and quantitative reverse transcription-polymerase chain reaction (qRT-PCR). We found eight genes showing significant changes between the two breeds. Based on a literature review, these genes were indicative of differences in extracellular structure density and differences in the potential to metabolize xenobiotic chemicals and lipids. The differentially expressed genes indicated that KNP has a lower extracellular structure density and a lower potential to metabolize xenobiotic chemicals than YS. PMID: 18945290 Overall design: Gene expression comparison between KNP and YS pigs grown at identical conditions. Sample size: n=4 (total n=8) Adipose tissue: backfat in the middle of pig. Age: KNP (7 month-old), YS (6 month-old)

《动物遗传学》（Anim Genet）2009年2月；第40卷第1期：115-118页，2008年10月17日在线发表。
题目：朝鲜本土猪（Korean native pig, KNP）与约克夏猪（Yorkshire, YS）脂肪组织的差异表达转录本
作者：Moon JK、Kim KS、Kim JJ、Choi BH、Cho BW、Kim TH、Lee CK
单位：韩国首尔高丽大学生命科学与生物技术学院生物技术系
本研究通过检测并比较朝鲜本土猪（KNP）与约克夏猪（YS）的脂肪组织转录本，以解析品种特异性的转录调控变化。本研究同时采用Affymetrix猪基因组芯片与定量反转录聚合酶链反应（quantitative reverse transcription-polymerase chain reaction, qRT-PCR）技术，最终筛选得到8个在两个品种间存在显著差异表达的基因。结合文献调研结果，上述差异基因提示两个品种在细胞外结构密度、外源性化学物质及脂质代谢潜能方面存在差异。差异表达基因分析结果显示，相较于约克夏猪，朝鲜本土猪的细胞外结构密度更低，外源性化学物质代谢潜能也更弱。
PubMed文献编号：18945290
实验整体设计：相同饲养条件下KNP与YS猪的基因表达差异比较
样本量：每组n=4，总样本量n=8
采集组织：猪背部中段的皮下脂肪（背膘）
猪龄：朝鲜本土猪为7月龄，约克夏猪为6月龄