Green Synthesis of Silver Nanoparticles from Plant Extracts for the Removal of Moss from Stone Heritage Buildings

1. Moss chlorophyll data
This dataset contains measurements of chlorophyll a concentration in moss samples collected from stone surfaces at Yunfeng Tunpu, treated with varying concentrations of silver nanoparticles (AgNPs) and benzalkonium chloride (BC). Measurements were taken on days 1, 15, and 30 using spectrophotometry at wavelengths of 665 nm and 649 nm, with concentrations calculated according to the standard formula. The data were used to evaluate the inhibitory effect of the antimicrobial agents on the photosynthetic capacity of the moss.

2. Moss Treatment Micrograph
This dataset comprises micrographs of moss samples after treatment with antimicrobial agents. The images illustrate changes in moss cell morphology and pigmentation (chlorophyll) before and after treatment, providing visual evidence of the damaging effects of the agents (e.g., silver nanoparticles) on the moss. These images serve as crucial visual documentation for assessing moss removal efficacy.

3.Scanning Electron Microscope (SEM) images

This dataset contains scanning electron microscope (SEM) images of silver nanoparticles (AgNPs) green-synthesized using aqueous and ethanolic extracts of four plants (cinnamon, clove, thyme, Agastache rugosa). The images reveal the morphology (predominantly spherical) and size distribution of the synthesized AgNPs. These data are a key component of the physical characterization of the nanoparticles.

4. Soluble protein data
This dataset records the soluble protein content in moss samples treated with different agents (AgNPs, BC). The protein content was measured using the Bradford method (Coomassie Brilliant Blue G-250) at a wavelength of 595 nm and is expressed as micrograms per gram of moss fresh weight (μg/g). This data was used to analyze the impact of antimicrobial agents on the metabolic activity of moss cells.

5.Species identification data
This dataset contains information and results related to the species identification of moss samples collected from stone heritage surfaces at Yunfeng Tunpu. It primarily includes morphological characteristics descriptions and photomicrographs obtained using stereomicroscopy and light microscopy, along with the final list of identified species (e.g., Brachythecium procumbens, Erythrodontium julaceum). Identification was performed with reference to "Flora Bryophytorum Sinicorum" and "Moss Flora of Guizhou".

1. 苔藓叶绿素数据集
本数据集涵盖了从云峰屯堡石材表面采集的苔藓样品的叶绿素a（chlorophyll a）浓度测定数据，这些样品经不同浓度的银纳米颗粒（silver nanoparticles, AgNPs）与苯扎氯铵（benzalkonium chloride, BC）处理。测定于第1、15、30天开展，采用分光光度法（spectrophotometry）在665 nm与649 nm波长下进行，浓度依据标准公式计算得出。本数据集用于评估抗菌剂对苔藓光合能力的抑制效应。

2. 苔藓处理显微图像数据集
本数据集包含经抗菌剂处理后的苔藓样品的显微图像。这些图像展示了处理前后苔藓细胞形态与色素（叶绿素）的变化，为银纳米颗粒等抗菌剂对苔藓的损伤效应提供了可视化证据，可作为评估苔藓清除效果的关键可视化档案。

3. 扫描电子显微镜（Scanning Electron Microscope, SEM）图像数据集
本数据集包含利用四种植物（肉桂、丁香、百里香、藿香）的水提与乙醇提取物绿色合成的银纳米颗粒（AgNPs）的扫描电子显微镜图像。这些图像展示了合成银纳米颗粒的形貌（以球形为主）与粒径分布情况，是纳米颗粒物理表征的核心组成部分。

4. 可溶性蛋白数据集
本数据集记录了经不同药剂（AgNPs、BC）处理的苔藓样品的可溶性蛋白含量。蛋白含量采用Bradford法（考马斯亮蓝G-250，Coomassie Brilliant Blue G-250）在595 nm波长下测定，结果以每克苔藓鲜重的微克数（μg/g）表示。本数据集用于分析抗菌剂对苔藓细胞代谢活性的影响。

5. 物种鉴定数据集
本数据集包含从云峰屯堡石质文物表面采集的苔藓样品的物种鉴定相关信息与结果。其主要内容包括：利用体视显微镜与光学显微镜获取的苔藓形态特征描述与显微照片，以及最终确定的物种名录（如匍枝青藓（Brachythecium procumbens）、卷叶赤藓（Erythrodontium julaceum））；物种鉴定参考了《中国苔藓植物志》（Flora Bryophytorum Sinicorum）与《贵州苔藓植物志》（Moss Flora of Guizhou）。