Radiation-induced Dmp53-dependent expression profile. Drosophila melanogaster

Embryos were collected, aged, mock-treated/treated with 40Gy gamma radiation, and allowed to recover for 1.5 hours. Targets from 3 sets of wild type (yw, w1118) and 2 sets of mutant (Dmp53NS) biological replicates were generated and the expression profiles were determined using Affymetrix Drosophila Genechip 1 arrays. Comparisons between the sample groups allow the identification of genes with radiation-responsive and Dmp53-dependent expression patterns. Keywords: stress response, genetic modification Overall design: 3 sets of paired control and irradiated Wild Type and 2 sets of paired control and irradiated Mutant (Dmp53NS) biological replicates were analyzed

收集胚胎并按发育时长进行龄期标定，将胚胎分为假处理组与40Gyγ射线辐照处理组，随后使各组胚胎恢复培养1.5小时。针对3组野生型（Wild Type，yw、w1118）及2组突变型（Dmp53NS）生物学重复（biological replicates）样本生成检测靶标，并采用Affymetrix果蝇基因芯片1阵列（Affymetrix Drosophila Genechip 1 arrays）检测其基因表达谱（expression profiles）。通过不同样本组间的比对分析，可鉴定出具有辐射响应且依赖Dmp53的基因表达模式的基因。关键词：应激响应、基因修饰。实验设计：共分析3组配对的野生型对照与辐照样本，以及2组配对的突变型（Dmp53NS）对照与辐照样本的生物学重复。