HCA Placental Infection Atlas

HCA Placental Infection Atlas

Self-renewing human trophoblast stem cells (hTSC) can give rise to major trophoblast lineages, including syncytiotrophoblast (SCT) and extravillous trophoblast (EVT). hTSC has facilitated the study of placental development and function in vitro. Here, we aim to utilise the hTSC line (HMDMC 20/0005) to study human placental infection and inflammation. This study will provide the opportunity to better understand the pathogenesis of human placenta in the event of infection and inflammation. In brief, differentiated hTSC will be infected with relevant pathogens and stimulated with a panel of inflammatory cytokines. We will use single-cell genomic and spatial approaches to characterise cellular heterogeneity in response to infection or cytokines stimulus. In parallel, we will investigate the interaction between iPSC-derived macrophages with different trophoblast lineages during the course of infection using single-cell genomic and spatial methods.

This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/EGA study EGAS00001004722

HCA胎盘感染图谱
具有自我更新能力的人滋养层干细胞（human trophoblast stem cells, hTSC）可分化为合体滋养层细胞（syncytiotrophoblast, SCT）与绒毛外滋养层细胞（extravillous trophoblast, EVT）等主要滋养层细胞谱系。hTSC已为体外研究胎盘发育与功能提供了关键研究模型。本研究拟采用hTSC细胞系（HMDMC 20/0005）开展人类胎盘感染与炎症相关研究，以深入解析感染及炎症状态下人胎盘的发病机制。具体而言，研究人员将对分化后的hTSC施加相关病原体感染，并以一组炎性细胞因子进行刺激；同时采用单细胞基因组学与空间组学技术，解析细胞对感染或细胞因子刺激的异质性应答特征。此外，本研究还将借助单细胞基因组学与空间组学方法，探究感染过程中诱导多能干细胞（induced pluripotent stem cells, iPSC）衍生的巨噬细胞与不同滋养层细胞谱系之间的相互作用。

本数据集属于预发布数据。如需了解惠康桑格研究所（Wellcome Trust Sanger Institute）共享的预发布数据的规范使用规则（含任何出版延期相关细则），请访问http://www.sanger.ac.uk/datasharing/ 查看EGA研究项目EGAS00001004722的相关信息。