Raw data to accompany the manuscript 'Data for Engineering Lipid Metabolism of Chinese Hamster Ovary (CHO) Cells for Enhanced Recombinant Protein Production' published in the Journal Data in Brief

This repository consists of the raw western blot, microscopy and mass spectrometry data to accompany the manuscript 'Data for Engineering Lipid Metabolism of Chinese Hamster Ovary (CHO) Cells for Enhanced Recombinant Protein Production' published in the Journal Data in Brief and associated with the article 'Engineering of Chinese hamster ovary cell lipid metabolism results in an expanded ER and enhanced recombinant biotherapeutic protein production' published in the journal Metabolic Engineering (see DOI: 10.1016/j.ymben.2019.11.007). The western blot raw file is associated with Figure 1a and 1b of the Data in Brief manuscript. The confocal microscopy raw image files (x3) are associated with Figure 1c of the Data in Brief manuscript. The mass spectrometry files are the raw data that refers to the samples presented in Figure 5 of the Data in Brief manuscript. Files are labelled as in the Data in Brief and Metabolic Engineering manuscripts. The file name structures is as follows; CHO-Controlpoolai Where 'a' represents replicate 'a' of three biological replicates and 'i' refers to mass spectrometry technical analysis 1 of 3 technical analyses of each replicate (thus for each cell pool or line there are three biological replicates that are each analysed in triplicate such that there are 9 raw mass spectrometry files for each cell pool or line). All the mass spectrometry files are found in the compressed (zip) file named mass_spectrometry_raw_files_archive.zip本仓库包含用于支撑两篇已发表论文的蛋白质免疫印迹（western blot）、显微成像及质谱（mass spectrometry）原始数据。其一为发表于《数据简报（Data in Brief）》的《工程中国仓鼠卵巢（Chinese Hamster Ovary, CHO）细胞脂质代谢以提升重组蛋白产量》，其二为发表于《代谢工程（Metabolic Engineering）》的《中国仓鼠卵巢细胞脂质代谢工程可扩大内质网并提升重组治疗性蛋白产量》，其DOI为10.1016/j.ymben.2019.11.007。蛋白质免疫印迹原始文件对应《数据简报》论文的图1a与图1b。共3份共聚焦显微成像（confocal microscopy）原始图像文件，对应《数据简报》论文的图1c。质谱原始数据对应《数据简报》论文图5中呈现的样本。所有文件的命名规则均遵循《数据简报》与《代谢工程》两篇论文的标注方式，文件名结构示例为：CHO-Controlpoolai。其中字母“a”代表3次生物学重复中的第a次重复，字母“i”代表每个生物学重复的3次技术重复中的第1次质谱技术分析——即每个细胞池或细胞株均包含3次生物学重复，每一次生物学重复均进行3次技术重复分析，因此每个细胞池或细胞株对应9份质谱原始数据文件。所有质谱原始数据均打包于压缩文件mass_spectrometry_raw_files_archive.zip中。