Dataset related to the article "An optimized MRM-based workflow of the L-arginine/nitric oxide pathway metabolites revealed disease- and sex-related differences in the cardiovascular field"

This record contains raw data related to the article "An optimized MRM-based workflow of the L-arginine/nitric oxide pathway metabolites revealed disease- and sex-related differences in the cardiovascular field" Clinical data indicate that low circulating L-homoarginine (HArg) concentrations are associated with cardiovascular (CV) disease, CV mortality, and all-cause mortality. A high number of LC-based analytical methods for the quantification of HArg, in combination with the L-arginine (Arg)-related pathway metabolites, have been reported. However, these methods usually consider a limited panel of analytes. Thus, in order to achieve a comprehensive picture of the Arg metabolism, we described an improved targeted metabolomic approach based on a multiple reaction monitoring (MRM) mass spectrometry method for the simultaneous quantification of the Arg/nitric oxide (NO) pathway metabolites. This methodology was then employed to quantify plasma concentrations of these analytes in a cohort of individuals with different grades/types of coronary artery disease (CAD) in order to increase knowledge about the role of HArg and its associated metabolites in the CV field. Our results showed that the MRM method here implemented is suitable for the simultaneous assessment of a wide panel of amino acids involved in the Arg/NO metabolic pathway, in plasma samples from patients with CV disease. Further, the findings highlighted an impairment of the Arg/NO metabolic pathway, and suggest a sex-dependent regulation of this metabolic route.本数据集包含与论文《基于多反应监测（multiple reaction monitoring，MRM）优化的L-精氨酸/一氧化氮通路代谢物分析流程揭示心血管领域疾病及性别相关差异》相关的原始数据。临床数据表明，循环中低水平的L-高精氨酸（L-homoarginine，HArg）浓度与心血管（cardiovascular，CV）疾病、心血管病死率及全因死亡率显著相关。目前已有大量基于液相色谱（liquid chromatography，LC）的分析方法，可用于定量检测HArg及相关的L-精氨酸（L-arginine，Arg）通路代谢物，但此类方法通常仅能覆盖有限的分析物组。为全面解析Arg代谢全貌，本研究开发了一种基于多反应监测（MRM）质谱法的改进型靶向代谢组学方法，可同时定量检测Arg/一氧化氮（nitric oxide，NO）通路代谢物。随后，研究团队采用该方法对一组患有不同分级/类型冠状动脉疾病（coronary artery disease，CAD）的队列人群的血浆中上述分析物浓度进行定量，以深化对HArg及其相关代谢物在心血管领域中作用的认知。研究结果表明，本研究采用的MRM方法可用于同时定量检测心血管疾病患者血浆样本中参与Arg/NO代谢通路的多种氨基酸。此外，本研究还揭示了Arg/NO代谢通路的功能损伤，并提示该代谢通路存在性别依赖性调控。