Labelling transcriptional response to dissociation in mouse hippocampus using scSLAMseq. Labelling transcriptional response to dissociation in mouse hippocampus using scSLAMseq

Transcriptional response to dissociation is known to cause artefacts in single cell data sets. We used 4sU to label newly made transcripts during dissociation in order to to later remove them from the data set Overall design: 4sU was added to the dissociation buffer while the hippocampi were enzymatically dissociated for 30 min.

众所周知，细胞解离过程中的转录应答会引发单细胞数据集的实验伪影问题。我们通过4sU在解离阶段对新合成的转录本进行标记，从而可在后续分析中将其从数据集中剔除。
实验整体设计：在对海马体进行30分钟酶解解离时，我们向解离缓冲液中加入了4sU。